1) Query for finding out different PPI interactions found to occur in the Hypothalamus

CONSTRUCT {

?s1 <http://purl.obolibrary.org/obo/BFO_0000066> <http://example.org/brain:Hypothalamus> .

<http://example.org/brain:Hypothalamus> <http://www.w3.org/2000/01/rdf-schema#subClassOf> <http://dbpedia.org/ontology/Brain> .

?s1 <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.uniprot.org/core/Interaction> .
}

WHERE {

?s1 <http://purl.obolibrary.org/obo/BFO_0000066> <http://example.org/brain:Hypothalamus> .

?s1 <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.uniprot.org/core/Interaction> .

<http://example.org/brain:Amygdala> <http://www.w3.org/2000/01/rdf-schema#subClassOf> <http://dbpedia.org/ontology/Brain> .

}limit 10
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2) Explanation of the query:

?s1 = represents what the user is interested in 

<http://purl.obolibrary.org/obo/BFO_0000066> = BFO ontology used to define the edge and the id BFO_0000066 denotes the relation "occurs in"

<http://example.org/brain:Hypothalamus> = Brain region of interest

<http://purl.uniprot.org/core/Interaction> = the user describes the unknown entry as a type of an interaction (PPI interaction to be more specific)

Finally we limit the number of interactions to 10 (for better visualization of the result)

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3) USE OF CONSTRUCT INSTEAD OF SELECT

CONSTRUCT is an alternative for the standard SELECT used in query language. The CONSTRUCT is used to return an RDF graph while SELECT command 
displays the result as a table